CaMKII Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90176-50, BS90176-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13554(Human) Q13555(Human) Q13557(Human) Q9UQM7(Human) P11798(Mouse) P28652(Mouse) Q6PHZ2(Mouse) Q923T9(Mouse) P08413(Rat) P11275(Rat) P11730(Rat) P15791(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CaMKII polyclonal antibody detects endogenous levels of CaMKII protein.

Bioworld Molecular Weight: 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CaMKⅡ on different lysates using anti-CaMKⅡ antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY-5Y
Lane 2: PC-12
Lane 3: SHG-44, ICC staining CaMKⅡ in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2A, CAMKA, KIAA0968, CaM kinase II subunit beta, CaMK-II subunit beta, CAMK2B, CAM2, CAMK2, CAMKB, CaM kinase II subunit gamma, CaMK-II subunit gamma, CAMK2G, CAMK, CAMK-II, CAMKG, Calcium/calmodulin dependent protein kinase II alpha, Calcium/calmodulin dependent protein kinase II beta, Calcium/calmodulin dependent protein kinase II delta, Calcium/calmodulin dependent protein kinase II gamma, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II alpha, CaM kinase II, CaM kinase II beta, CaM kinase II delta, CaM kinase II gamma, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2, Camk2a, CAMK2B, CAMK2D, CAMK2G, CAMKA, KCC2A,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified