CD21 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90226-50, BS90226-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P20023(Human) P19070(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC, IHC, IP, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: CD21 is a type I integral membrane glycoprotein that serves as a receptor for the C3d complement fragment and for the Epstein-Barr virus. It plays a role in B cell activation and proliferation and undergoes phosphorylation after B cell activation with phorbol esters. CD21 is expressed on mature B cells, follicular dendritic cells, pharyngeal and cervical epithelial cells and a subset of thymocytes. The adaptive immune response is tightly regulated to limit responding cells in an antigen-specific manner. On B cells, co-receptors CD21/CD19 modulate the strength of B cell Ag receptor (BCR) signals, thereby influencing cell fate. Complement receptor (CR) type 2 (CR2/ CD21) is normally expressed during the immature and mature stages of B cell development. In association with CD19, CR21 plays an important role in enhancing mature B cell responses to foreign antigens.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CD21 polyclonal antibody detects endogenous levels of CD21 protein.

Bioworld Molecular Weight: 130-155 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD21 on different lysates using anti-CD21 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Daudi
Lane 2: Raji, ICC staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Cr2, EBV receptor, CR2, C3DR, CD 21, CD21, Complement C3d receptor, Complement component (3d/Epstein Barr virus) receptor 2, Complement receptor type 2, CR, Cr2, CR2, CVID7, EBV receptor, EBV-R, Epstein Barr virus receptor, Epstein-Barr virus receptor, EVBR, SLEB9,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified