CD27 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90228-50, BS90228-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P26842(Human)

Host: Rabbit

Reactivity: Human, rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
IHC:1:50-1:200
ICC:1:50-1:200
FC:1:50-1:100

Background: The tumor necrosis factor (TNF) receptor family is composed of several type I integral membrane glycoproteins that exhibit homology in their cysteine-rich extracellular domains. Members of this family include FAS, OX40, CD27 and CD30. Ligands for these receptors are often type II transmembrane glycoproteins, as is the case for CD27 and CD30. CD27 is a homodimeric lymphocyte-specific surface antigen present on T and B lymphocytes. Activation of the CD3 complex via the T cell receptor for antigen leads to an increase in CD27 expression. Together, CD27 and its ligand, CD27L, generate co-stimulatory signals required for complete T cell activation. CD30 is a surface marker for neoplastic cells of the Hodgkin's lymphoma and related hematologic malignancies. CD30L has been shown to enhance the proliferation of the Hodgkin's cell line HDLM-2, but exerts antiproliferative effects on large cell anaplastic lymphoma cell lines.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CD27 polyclonal antibody detects endogenous levels of CD27 protein.

Bioworld Molecular Weight: Predicted band size: 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD27 on Raji cell using anti-CD27 antibody at 1/500 dilution., ICC staining CD27 in K562 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TNFRSF7, CD 27, CD27, CD27 antigen, CD27 molecule, CD27, CD27L receptor, LPFS2, MGC20393, OTTHUMP00000238557, S152, T cell activation antigen CD27, T cell antivation antigen S152, T-cell activation antigen CD27, T14, TNFRSF 7, TNFRSF7, TNFSF7, Tp 55, Tp55, Tumor necrosis factor receptor superfamily member 7,

Immunogen: Intracellular domain corresponding to Human CD27.

Conjugate: Unconjugated

Modification: Unmodified