ICAM-1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9943M-50, BS9943M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05362

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:5000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200

Background: ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase in inflammation. Also, ICAM1 is detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of ICAM-1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 58, 96 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ICAM-1 Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HUVEC whole cell lysate
Lane 3: Raji whole cell lysate, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody.Counter stained with hematoxylin., ICC staining ICAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54; ICAM1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified