PTGS1 (S585) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1075-50, BS1075-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23219

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:50~1:200

Background: Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid. While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression. Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock. Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PTGS1 (S585) polyclonal antibody detects endogenous levels of PTGS1 protein.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PTGS1 (S585) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: Rat kidney tissue lysate, Immunohistochemistry (IHC) analyzes of PTGS1 (S585) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50., Immunoprecipitation of EC9706 cell lysates using PTGS1 (S585) pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using PTGS1 (S585) pAb.

Alternative Name: Cyclooxygenase-1; COX-1; Prostaglandin-endoperoxide synthase 1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; PTGS1; COX1

Immunogen: Synthetic peptide, corresponding to amino acids 555-600 of Human PTGS1.

Conjugate: Unconjugated

Modification: Unmodified