RGS10 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS5895-50, BS5895-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43665

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. In mammals, G protein α, β and γ polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their α subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four Gα GTPase-activating proteins (GAPs) have been identified and are designated RGS1 (regulator of G protein signaling), RGS4, RGS10 and GAIP (Gα-interacting protein). Each of these proteins has been shown to deactivate specific Gα isoforms by increasing the rate at which they convert GTP to GDP. RGS1, RGS4 and GAIP bind tightly to and exhibit GAP activity towards Gα i, Gα o and Gα t, but not Gα s. RGS10 increases the GTP hydrolytic activity of several members of the Gα i sub-family, including Gα i-3, Gα z and Gα o.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RGS10 polyclonal antibody detects endogenous levels of RGS10 protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RGS10 polyclonal antibody at 1:500 dilution
Lane 1: Jurkat whole cell lysate (40µg)
Lane 2: Myla2059 whole cell lysate (40µg)
Lane 3: HUT78 whole cell lysate (40µg)
Lane 4: K562 whole cell lysate (40µg)
Lane 5: SGC7901 whole cell lysate (40µg), Immunohistochemistry (IHC) analyzes of RGS10 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of RGS10 pAb in paraffin-embedded human stomach carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Regulator of G-protein signaling 10

Immunogen: Synthetic peptide, corresponding to amino acids 357-402 of Human RGS10.

Conjugate: Unconjugated

Modification: Unmodified