ATP5I (K69) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3813-50, BS3813-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P56385

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP 1:50 - 1:100

Background: Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. The two complexes are linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled, via a rotary mechanism of the central stalk subunits, with proton translocation across the membrane. ATP5I, also known as mitochondrial ATP synthase subunit E or ATP5K, is a 69 amino acid protein member of the ATPase E subunit family. Localized to the inner membrane of the mitochondria, ATP5I is a part of the F0 complex.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATP5I (K69) polyclonal antibody detects endogenous levels of ATP5I protein.

Bioworld Molecular Weight: ~ 8 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATP5I (K69) pAb at 1:500 dilution
Lane 1: AML-12 whole cell lysate (40ug)
Lane 2: C6 whole cell lysate (40ug)
Lane 3: HepG2 whole cell lysate (40ug)
Lane 4: PC3 whole cell lysate (40ug)
Lane 5: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ATP5I (K69) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of HEK293T cell lysate using ATP5I (K69) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using ATP5I (K69) #BS3813. “↑”（supernatant); “↓(deposition）

Alternative Name: ATP synthase subunit e, mitochondrial; ATPase subunit e; ATP5I; ATP5K

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human ATP5I.

Conjugate: Unconjugated

Modification: Unmodified