PCNA Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66679-50, MB66679-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P12004

Host: Mouse

Reactivity: Human, Mouse, Rat, Monkey, Hamster

Applications: WB, IHC, IF/ICC, IP,

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Background: Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of PCNA protein.

Bioworld Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PCNA expression in Hela (A), NIH3T3 (B), COS7 (C), C6 (D), CHOK1 (E) whole cell lysates., Immunohistochemical analysis of PCNA staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.69). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Alternative Name: Proliferating cell nuclear antigen; PCNA; Cyclin

Immunogen: Purified recombinant human PCNA protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified