Collagen 5 alpha 2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67467-50, BS67467-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P05997

Host: Rabbit

Reactivity: Human, Rat, Pig

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Background: The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue,such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Collagen 5 alpha 2 protein.

Bioworld Molecular Weight: ~ 128 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Collagen 5 alpha 2 expression in H9C2 (A), PC3 (B), MCF7 (C) whole cell lysates., Immunohistochemical analysis of Collagen 5 alpha 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of Collagen 5 alpha 2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: Collagen alpha-2(V) chain

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 5 alpha 2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified