CD142 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0267P-50, NCP0267P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P13726

Host: Rabbit

Reactivity: Human

Applications: WB, IP, IF

All Applications: WB: 1:2000~1:5000
IF 1:50~1:200
IP 1:50 - 1:200

Background: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD142 polyclonal antibody detects endogenous levels of CD142 protein.

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD142 polyclonal antibody at 1:2000 dilution
Lane 1: A549 cell membrane lysate (25ug)
Lane 2: L02 cell membrane lysate (25ug)
Lane 3: EC9706 cell membrane lysate (25ug), Immunofluorescence analysis of A549 cells using CD142 pAb at dilution of 1:200., Immunoprecipitation of SK-OVCAR3 cell membrane lysates using CD142 pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using CD142 pAb.

Alternative Name: Tissue factor; TF; Coagulation factor III; Thromboplastin; CD142

Immunogen: Recombinant protein of human CD142.

Conjugate: Unconjugated

Modification: Unmodified