Claudin 7 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66138-50, MB66138-100

Product: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: O95471

Host: Mouse

Reactivity: Human

Applications: WB, IHC

All Applications: WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Background: The Claudin superfamily consists of many structurally related proteins in humans . These proteins are important structural and functional components of tight junctions in paracellular transport . Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues . Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and Junction adhesion molecule . Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands . Claudin expression is highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions . mRNA studies indicate that Claudin-7 is specifically expressed in mouse lung and kidney, but not in heart, brain, spleen, liver, skeletal muscle or testis . The gene encoding human Claudin-7 maps to chromosome 17p13 .

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Claudin 7 protein.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Claudin 7 expression in LNCaP (A) whole cell lysates., Immunohistochemical analysis of Claudin 7 staining in human chromophobe cell renal carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Claudin 7 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Claudin 7 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: CEPTRL2; CPETRL2; Claudin-7; CLDN-7

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Claudin 7. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified