AGRN Polyclonal Antibody

Catalogue Numbers: BS62062-50, BS62062-100

Sizes: 50µl, 100µl

Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Swiss-Prot: O00468

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated “y” and “z” display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle .

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: AGRN polyclonal antibody detects endogenous levels of AGRN protein.

Extra Notes: Western blot (WB) analysis of AGRN polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate(40µg)
Lane2:H1792 whole cell lysate(40µg)
Lane3:PC12 whole cell lysate(40µg)
Lane4:AML-12 whole cell lysate(40µg)

Bioworld Molecular Weight: ~ 217 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Agrin; Cleaved into the following 4 chains: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90; Agrin C-terminal 22 kDa fragment; C22; AGRN; AGRIN

Immunogen: Synthetic peptide, corresponding to Human AGRN

Conjugate: Unconjugated

Modification: Unmodified