cGK 2 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67256-50, BS67256-100

Sizes: 50µl, 100µl

Swiss-Prot: Q13237

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: Crucial regulator of intestinal secretion and bone growth (By similarity).
Phosphorylates and activates CFTR on the plasma membrane. Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum (By similarity).
Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863 (By similarity).
Acts as regulator of gene expression and activator of the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2 in mechanically stimulated osteoblasts. Under fluid shear stress, mediates ERK activation and subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2 and FOSB that play a key role in the osteoblast anabolic response to mechanical stimulation (By similarity).

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 217% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of cGK 2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of cGK 2 expression in A549 (A), NIH3T3 (B), HEK293T (C) whole cell lysates., Immunohistochemical analysis of cGK 2 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 13 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: PRKGR2; cGMP-dependent protein kinase 2; cGK 2; cGK2; cGMP-dependent protein kinase II; cGKII

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human cGK 2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified