H2-Aa Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77661-50, BS77661-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P14434

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Enables peptide antigen binding activity. Involved in response to interferon-gamma. Acts upstream of or within antigen processing and presentation of exogenous peptide antigen via MHC class II and positive regulation of T cell differentiation. Located in external side of plasma membrane and lysosome. Part of MHC class II protein complex. Is expressed in central nervous system; retina; and thymus primordium. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); glomerulonephritis (multiple); hematologic cancer (multiple); and systemic scleroderma (multiple). Orthologous to several human genes including HLA-DQA1 (major histocompatibility complex, class II, DQ alpha 1).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using H2-Aa antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 28KDa

Alternative Name: Ia1; H2Aa; Ia-1; H-2Aa; Aalpha; IAalpha; I-Aalpha; H2-Aa

Immunogen: Recombinant fusion protein of mouse H2-Aa(NP_034508.2).

Conjugate: Unconjugated

Modification: Unmodified