CD32 (FcgammaRIIb) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0200P-50, NCP0200P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P31994

Host: Rabbit

Reactivity: Human

Applications: WB, IF

All Applications: WB: 1:1000~1:2000
IF 1:50~1:200

Background: Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD32 (FcgammaRIIb) polyclonal antibody detects endogenous levels of CD32 protein.

Bioworld Molecular Weight: ~ 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD32 (FcgammaRIIb) polyclonal antibody at 1:1000 dilution
Lane 1: SGC7901 whole cell lysate (30ug)
Lane 2: EC9706 cell membrane lysate (24ug), Immunofluorescence analysis of SGC7901 cells using CD32 (FcgammaRIIb) pAb at dilution of 1:200.

Alternative Name: Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD32; FCGR2B; CD32; FCG2; IGFR2

Immunogen: Recombinant protein of human CD32.

Conjugate: Unconjugated

Modification: Unmodified