APE1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90069-50, BS90069-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P27695(Human) P28352(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:500-1:1,000
IHC:1:100-1:500
FC:1:50-1:100

Background: The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: APE1 polyclonal antibody detects endogenous levels of APE1 protein.

Bioworld Molecular Weight: 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of APE1 on different lysates using anti-APE1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HL-60
Lane 2: Human skin tissue
Lane 3: MCF-7
Lane 4: Mouse placenta tissue, ICC staining APE1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: APEN, AP endonuclease 1, APE-1, APEX1, APE, APE1, APEX, APX, HAP1, REF1, AP endonuclease 1, AP endonuclease class I, AP lyase, APE 1, APE, APE-1, APEN, APEX 1, APEX, APEX nuclease (multifunctional DNA repair enzyme) 1, Apex nuclease 1, APEX nuclease, APEX1, APEX1, Apurinic endonuclease, Apurinic-apyrimidinic endonuclease 1, Apurinic/apyrimidinic (abasic) endonuclease, Apurinic/apyrimidinic endonuclease 1, Apurinic/apyrimidinic exonuclease, APX, BAP1, Deoxyribonuclease (apurinic or apyrimidinic), DNA (apurinic or apyrimidinic site) lyase, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial, EC 4.2.99.18, HAP 1, HAP1, Human Apurinic endonuclease 1, MGC139790, Multifunctional DNA repair enzyme, Redox factor 1, Redox factor-1, REF 1, REF 1 protein, REF-1, REF1, REF1 protein,

Immunogen: Recombinant protein with Human APE1 aa 1-250.

Conjugate: Unconjugated

Modification: Unmodified