[KO Validated] MTCH2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS72216-50, BS72216-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y6C9

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF/ICC

All Applications: WB,1:1000 - 1:3000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the SLC25 family of nuclear-encoded transporters that are localized in the inner mitochondrial membrane. Members of this superfamily are involved in many metabolic pathways and cell functions. Genome-wide association studies in human have identified single-nucleotide polymorphisms in several loci associated with obesity. This gene is one such locus, which is highly expressed in white adipose tissue and adipocytes, and thought to play a regulatory role in adipocyte differentiation and biology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA can give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 33KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts from wild type and MTCH2 knockout HeLa cells, using MTCH2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s., Immunofluorescence analysis of NIH-3T3 cells using MTCH2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-2 OS cells using MTCH2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: MTCH2; HSPC032; MIMP; SLC25A50

Immunogen: Recombinant fusion protein of human MTCH2(NP_055157.1).

Conjugate: Unconjugated

Modification: Unmodified