COX7A2/3 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67060-50, BS67060-100

Sizes: 50µl, 100µl

Swiss-Prot: O60397; P14406

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Cytochrome c oxidase, the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of three catalytic subunits encoded by mitochondrial genes, and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, while the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (liver isoform) of subunit VIIa, with this polypeptide being present in both muscle and non-muscle tissues. In addition to polypeptide 2, subunit VIIa includes polypeptide 1 (muscle isoform), which is present only in muscle tissues, and a related protein, which is present in all tissues. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4 and 14

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of COX7A2/3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of COX7A2/3 expression in HepG2 (A), Jurkat (B) whole cell lysates., Immunohistochemical analysis of COX7A2/3 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 12 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: COX7A2P2; COX7A3; COX7AL2; COX7AP2; Putative cytochrome c oxidase subunit 7A3 mitochondrial; Cytochrome c oxidase subunit VIIa 3; COX7A2; COX7AL; Cytochrome c oxidase subunit 7A2 mitochondrial; Cytochrome c oxidase subunit VIIa-liver/heart; Cytochrome c oxidase subunit VIIa-L; Cytochrome c oxidase subunit VIIaL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human COX7A2/3. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified