RPL8 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67336-50, BS67336-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P62917

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/2000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Background: Component of the large ribosomal subunit.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of RPL8 protein.

Bioworld Molecular Weight: ~ 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RPL8 expression in HepG2 (A), MCF7 (B), mouse lung (C), rat liver (D) whole cell lysates., Immunohistochemical analysis of RPL8 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of RPL8 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: 60S ribosomal protein L8

Immunogen: Recombinant full length protein of human RPL8

Conjugate: Unconjugated

Modification: Unmodified