ELL (D125) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2020-50, BS2020-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P55199

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: ELL was shown to encode an elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. ELL is the second elongation factor to be implicated in oncogenesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ELL (D125) polyclonal antibody detects endogenous levels of ELL protein.

Bioworld Molecular Weight: ~ 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ELL (D125) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate, Immunohistochemistry (IHC) analyzes of ELL (D125) pAb in paraffin-embedded human liver carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ELL (D125) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: RNA polymerase II elongation factor ELL; Eleven-nineteen lysine-rich leukemia protein; C19orf17

Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human ELL.

Conjugate: Unconjugated

Modification: Unmodified