Acetyl-Histone H4-K8 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74016-50, BS74016-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P62805

Host: Rabbit

Reactivity: Human, Mouse, Rat, Other (Wide Range)

Applications: WB, IHC, IF/ICC, IP, , ChIP, , ChIP, -seq

All Applications: WB,1:1000 - 1:3000 | IHC,1:200 - 1:500 | IF/ICC,1:500 - 1:1000 | IP,1:200 - 1:500 | ChIP,1:20 - 1:50 | ChIP-seq,1:20 - 1:50

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Acetylated Antibodies

Bioworld Molecular Weight: 11kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunohistochemistry of paraffin-embedded human kidney using Acetyl-Histone H4-K8 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse liver using Acetyl-Histone H4-K8 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat kidney using Acetyl-Histone H4-K8 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: FO108; H4; H4/n; H4F2; H4FN; HIST2H4; Histone H4; HIST1H4A; HIST2H4A

Immunogen: A synthetic acetylated peptide around K8 of human Histone H4(NP_003529.1).

Conjugate: Unconjugated

Modification: Acetylated