The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.

ROTI^®Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose.

ROTI^®Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI^®Load DNA. ROTI^®Load DNA gel loading buffer is usually used as 1x concentrated solution.

ROTI^®Load DNA small (with glycerol) s a special gel loading buffer for gel electrophoresis of small fragments in which only xylene cyanol was used as dye. The buffer thus efficiently avoids the covering of important DNA bands in the small fragment area and enables a clean presentation, even in highly concentrated agarose gels (see lane 3 in the product image).

Glycerin is the standard reagent for increasing sample density.

Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.